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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be observed in many tumors, which results in chromosome instability, and so might lead to tumorigenesis. This protein was observed to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical job in selling mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already described. Two extra splice variants were being noted but specific nucleotide sequence information and facts is not really nonetheless readily available.

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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide generally has the epitope recognized because of the antibody.

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This guideline outlines even more feasible optimization of cellular and immunoassay parameters to ensure the very best final results are...

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This manual outlines further more achievable optimization of mobile and immunoassay parameters to make certain the absolute best success are...

Antibodies bound to the blocking peptide not bind into the epitope on the target protein. By evaluating the staining from the blocked antibody as opposed to the antibody on your own, one can see which staining is unique.

Target Information Cyclin E belongs on the highly conserved cyclin spouse and children, whose members are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins functionality as regulators of CDK kinases. Diverse cyclins exhibit unique expression and degradation patterns which lead towards the temporal coordination of every mitotic party. Cyclin E types a complex with and capabilities to be a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates at the G1-S section boundary and is particularly degraded as cells development by S period.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...

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